Cell line/type | Whole embryo |
---|---|
Species | Bovine (cow) |
Animal free | No |
Product | PES-CDM |
Barceló‐Fimbres, M., & Seidel Jr, G. E. (2007). Effects of fetal calf serum, phenazine ethosulfate and either glucose or fructose during in vitro culture of bovine embryos on embryonic development after cryopreservation. Molecular Reproduction and Development: Incorporating Gamete Research, 74(11), 1395-1405. This study investigated the effects of hexoses, fetal calf serum (FCS), and phenazine ethosulfate (PES) during the culture of bovine embryos on blastocyst development and survival after cryopreservation by slow freezing or vitrification. The basal, control medium was chemically defined (CDM) plus 0.5% fatty acid-free BSA. Replacement of glucose with fructose resulted in higher in vitro blastocyst production, but no differences were found for this response between PES, FCS, and control groups. Cryopreservation by vitrification and conventional freezing resulted in similar survival rates of blastocysts. Reduction of cytoplasmic lipid content of bovine embryos by culturing them with PES improved cryotolerance relative to controls cultured without serum. Embryos cultured with the presence of FCS had poorer survival post-cryopreservation than controls or the PES treatment. The formulation of PES-CDM is listed in the Materials and Methods, under the heading; Culture of embryo's. The formulation of the basal medium CDM is listed in Table 1 of a previous article, De La Torre-Sanchez et al. 2006b. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |