Cell line/type | Whole embryo |
---|---|
Species | Bovine (cow) |
Animal free | No |
Product | mSOF-Atra |
Gómez, E., Rodríguez, A., Muñoz, M., Caamaño, J. N., Carrocera, S., Martín, D., ... & Díez, C. (2008). Development and quality of bovine morulae cultured in serum-free medium with specific retinoid receptor agonists. Reproduction, Fertility and Development, 20(8), 884-891. In this study, the effect of short-term exposure (24 h) to retinoids on in vitro-produced bovine morulae is analyzed. Immature cumulus-oocyte complexes were in vitro matured and fertilized. Presumptive zygotes were subsequently cultured in modified synthetic oviduct fluid up to Day 6, in which morulae were randomly allocated to the different experimental groups. The treatments consisted of 0.1 microM LG100268 (LG; a retinoid X receptor agonist), 0.7 microM all-trans retinoic acid (ATRA; a retinoic acid receptor agonist) or no additives. Day 8 blastocyst development was increased in the ATRA-treated group compared with the LG and untreated embryos. In Day 7 embryos, the number of total cells and cells allocated to the trophectoderm were higher in the ATRA-treated group compared with untreated embryos. Apoptosis in the inner cell mass increased after LG treatment, whereas ATRA had no effect. After vitrification and warming, survival and hatching rates of Day 7 blastocysts did not change with retinoid treatment. Within the LG-treated and untreated blastocyst groups, survival and hatching rates were higher for Day 7 than Day 8 embryos; however, Day 8 blastocysts treated with ATRA showed improved hatching rates. In conclusion, treatment of morulae with ATRA in serum-free medium improves embryo development and quality without increasing the incidence of apoptosis and necrosis. The composition of mSOF + Altra is specified in the Materials and Methods, under the heading; In vitro embryo culture. Formulation of mSOF is described by Holm et al. 1999. Holm, P., Booth, P. J., Schmidt, M. H., Greve, T., and Callesen, H. (1999). High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myoinositol http://www.publish.csiro.au/rd/pdf/RD08103 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |