Cell line/type | Whole embryo |
---|---|
Species | Bovine (cow) |
Animal free | No |
Product | SOFaa-BSA-ITS Wydooghe et al. 2014 |
Wydooghe, E., Heras, S., Dewulf, J., Piepers, S., Van den Abbeel, E., De Sutter, P., ... & Van Soom, A. (2014). Replacing serum in culture medium with albumin and insulin, transferrin and selenium is the key to successful bovine embryo development in individual culture. Reproduction, Fertility and Development, 26(5), 717-724. In this study was determined whether the timing of removal of serum (during maturation or culture) had an influence on individual embryo development. The results clearly showed that removal of serum during embryo culture was the main contributing factor since high blastocyst development was observed after individual culture in synthetic oviductal fluid supplemented with bovine serum albumin (BSA) and insulin, transferrin and selenium (ITS), independent of the maturation medium. Second, we investigated whether an individual factor of the ITS supplement was essential for individual embryo development. It was demonstrated that repeatable high blastocyst percentages were due to the synergistic effect of ITS. The data showed that individual culture in serum-free conditions leads to high blastocyst development, but group culture still improves blastocyst quality. Oocytes were matured either in serum or in serum-free TCM-199. Serum-free TCM-199 was supplemented with different components as specified in the Materials and Methods, under the heading; In vitro embryo production. After fertilization, zygotes were cultured in serum or in serum-free SOFaa with different components as specified in the Materials and Methods, under the heading; In vitro embryo production. http://www.publish.csiro.au/rd/pdf/RD13043 |
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Source | Literature - own formulation |
Chemically defined > Unspecified | Semi-defined |
Contains phenol red > Unspecified | Optional |
Antibiotics free > Yes | Yes |