Cell line/type | Whole embryo |
---|---|
Species | Bovine (cow) |
Animal free | Yes |
Product | Two-step culture medium Jang et al. 2011 |
Jang, G., Hong, S. G., & Lee, B. C. (2011). Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid. Journal of veterinary science, 12(1), 83-89. In this study, the productivity of calves resulting from embryos derived with different culture media was determined. Enucleated oocytes matured in vitro were reconstructed with fetal fibroblasts, fused, and activated. The cloned embryos were cultured in modified synthetic oviduct fluid (mSOF) or a chemically defined medium (CDM) and developmental competence were monitored. These results demonstrate that SCNT embryos cultured in CDM showed higher viability as judged by survival of the calves that came to term compared to blastocysts derived from mSOF cultures. The CDM, two-step culture medium, has been described previously in Lim et al. 2007, Table 1. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |