Cell line/type | Whole embryo |
---|---|
Species | Bovine (cow) |
Animal free | Yes |
Product | SOF-PVA (CDC) |
Gómez, E., Muñoz, M., Simó, C., Ibáñez, C., Carrocera, S., Martín-González, D., & Cifuentes, A. (2016). Non-invasive metabolomics for improved determination of embryonic sex markers in chemically defined culture medium. Journal of Chromatography A, 1474, 138-144. In this study, a single bovine embryo culture step for 24h supported improved birth rates under chemically defined conditions (CDC) to investigate biomarker detection of embryonic sex in contrast to classical BSA-containing medium. In vitro matured slaughterhouse oocytes were fertilized in vitro with a single bull. Embryos were initially cultured in synthetic oviduct fluid with BSA. On day-6, morulae were cultured individually in droplets with (BSA) or without protein (CDC). Both systems, i.e. CDC and the BSA-containing medium, which is more similar to the physiological situation, showed significant differential concentrations of the same 6 molecules, but only CDC was effective. The precise formulation of CDC, the SOF-PVA containing medium, is listed in the Appendix A Supplementary data. In Supplementary Table S1, the composition of synthetic oviduct fluid (SOF) medium is listed, to which macromolecular supplements (i.e. BSA or PVA) were added in this study. https://ac.els-cdn.com/S0021967316314625/1-s2.0-S0021967316314625-main.pdf?_tid=ed4298e6-39ea-4691-aebc-e10310f11437&acdnat=1530020064_f36bbcb900bf17546963386c3b13657b |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |