|Cell line/type||Whole embryo|
|Product||Two-step culture media|
Lim, K. T., Jang, G., Ko, K. H., Lee, W. W., Park, H. J., Kim, J. J., & Kang, S. K. (2007). Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined media. Theriogenology, 67(2), 293-302.
In this study, the authors developed a defined culture medium that supported improved in vitro bovine embryo development and calving rate after embryo transfer (ET). In vitro-matured bovine oocytes from abbatoir-derived ovaries from Korean native, HanWoo cattle were fertilized with frozen-thawed spermatozoa and embryos were cultured in two-step culture media. Chemically defined two-step culture media without BSA or FBS improved developmental competence of in vitro cultured bovine embryos and delivery of viable calves after embryo transfer.
The formula of two-step culture media, both early stage medium and later stage medium, are listed in Table 1.https://www.sciencedirect.com/science/article/pii/S0093691X06004171
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > Yes||Yes|