|Cell line/type||Whole embryo|
Okuyama, M., & Funahashi, H. (2012). Glycosaminoglycans improves early development of zona-free 8-cell rat embryos to blastocysts in a chemically defined medium, but not the pregnancy rate following transfer of the blastocysts. Journal of Reproduction and Development, 58(3), 295-301.
The objective of the present study was to clarify the possible role of the zona pellucida (ZP) in the early development of rat embryos and to determine the effect of glycosaminoglycans on the development of ZP-free 8-cell embryos before or after embryo transfer at the blastocyst stage. These embryos were cultured in a chemically defined mR1ECM for 24 h.
The formula of mR1ECM medium is listed in Table 1.https://www.jstage.jst.go.jp/article/jrd/58/3/58_11-092H/_pdf
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > Yes||Yes|