Cell line/type | Whole embryo |
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Species | Goat |
Animal free | Yes |
Product | DI/II medium |
Choi, W. J., Lee, J. H., Lee, S. H., Yum, S. Y., Lee, S. J., Lim, C. W., & Jang, G. (2016). Developmental competence and cryotolerance of caprine parthenogenetic embryos cultured in chemically defined media. Theriogenology, 86(2), 596-603. In this study, the authors demonstrated the in vitro culture of domestic goat (Capra hircus) embryos using two types of culture media, modified synthetic oviductal fluid (mSOF) and a two-step chemically defined medium (DI/II). Overall, the data reported that the defined in vitro culture media for goat embryos were established with high efficiency. Using a defined medium for goat embryos showed a significantly increased blastocyst formation rate and cell number compared with the BSA-containing medium. Although the cleavage rates of embryos cultured in mSOF and DI/II were not significantly different (96.6% in mSOF and 95.4% in DI/II), a significantly higher number of blastocysts were developed in DI/II compared with mSOF (64.6% in mSOF and 82.3% in DI/II; and blastocyst formation was observed). The formulation of DI/II medium is presented in a previous study, Lim et al., 2007. https://www.sciencedirect.com/science/article/pii/S0093691X16000844 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |