|Cell line/type||Whole embryo|
Choi, W. J., Lee, J. H., Lee, S. H., Yum, S. Y., Lee, S. J., Lim, C. W., & Jang, G. (2016). Developmental competence and cryotolerance of caprine parthenogenetic embryos cultured in chemically defined media. Theriogenology, 86(2), 596-603.
In this study, the authors demonstrated the in vitro culture of domestic goat (Capra hircus) embryos using two types of culture media, modified synthetic oviductal fluid (mSOF) and a two-step chemically defined medium (DI/II). Overall, the data reported that the defined in vitro culture media for goat embryos were established with high efficiency. Using a defined medium for goat embryos showed a significantly increased blastocyst formation rate and cell number compared with the BSA-containing medium. Although the cleavage rates of embryos cultured in mSOF and DI/II were not significantly different (96.6% in mSOF and 95.4% in DI/II), a significantly higher number of blastocysts were developed in DI/II compared with mSOF (64.6% in mSOF and 82.3% in DI/II; and blastocyst formation was observed).
The formulation of DI/II medium is presented in a previous study, Lim et al., 2007.
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > Yes||Yes|