Cell line/type | Whole embryo |
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Species | Human |
Animal free | Yes |
Product | Global-Medium and MultiBlast Medium |
Kawamura, K., Chen, Y., Shu, Y., Cheng, Y., Qiao, J., Behr, B., ... & Hsueh, A. J. (2012). Promotion of human early embryonic development and blastocyst outgrowth in vitro using autocrine/paracrine growth factors. PLoS One, 7(11), e49328. In this study, combined treatment with key embryonic growth factors (brainderived neurotrophic factor, colony-stimulating factor, epidermal growth factor, granulocyte macrophage colonystimulating factor, insulin-like growth factor-1, glial cell-line derived neurotrophic factor, and artemin) in serum-free media promoted .2.5-fold the development of tri-pronuclear zygotes to blastocysts. Tri-pronuclear zygotes were cultured in Global-Medium (G-M, LifeGlobal, Guilford, CT) with 5% human serum albumin in the presence or absence of EGF, IGF-I, GM-CSF, BDNF, and CSF-1, all at 10 ng/ml. Embryos cultured in MultiBlast Medium (Irvine Scientific, Santa Ana, CA) with 10% Serum Substitute Supplement (SSS, Irvine Scientific, Santa Ana, CA) and further cultured at 37uC with 5% CO2, 5% O2 and 90% N2 with or without growth factor mixtures containing 10 ng/ml of EGF, IGFI, GM-CSF, BDNF, CSF-1, artemin, and GDNF (R&D Systems). Global-Medium and MultiBlast Medium are commercial products. Their formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3495911/pdf/pone.0049328.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |