Cell line/type | Whole embryo |
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Species | Porcine (Pig) |
Animal free | Yes |
Product | POM+PVA |
Yoshioka, K., Suzuki, C., & Onishi, A. (2008). Defined system for in vitro production of porcine embryos using a single basic medium. Journal of Reproduction and Development, 54(3), 208-213. In this study, the effects of basic media and macromolecular components on in vitro maturation (IVM) were investigated to develop a defined system for in vitro embryo production using a single basic medium through IVM, IVF, and IVC. Porcine immature oocytes were matured in porcine oocyte medium (POM) or modified North Carolina State University (mNCSU) 37, which were supplemented with either 10% (v/v) porcine follicular fluid (pFF) or 3 mg/ml polyvinyl alcohol (PVA) as a macromolecular component (designated POM+pFF, POM+PVA, mNCSU37+pFF and mNCSU37+PVA). The normal fertilization rate was significantly higher in POM+PVA compared with the other treatments, although the total number of cells in blastocysts was reduced with the addition of PVA to both POM and mNCSU37 compared with pFF supplementation. These results demonstrate that porcine blastocysts can be produced by the defined system using a single basic medium. In conclusion, this study describes successful IVP fo porcine blastocysts in a defined system using a single basic medium. The use of a defined IVP system yields higher reproducibility of results and ensures the biosafety of culture by eliminating protein preparations, which may be contaminated with pathogens. The total number of cells in blastocysts were reduced by the use of PVA instead of pFF, although the percentage of embryos that developed to blastocysts after IVF did not differ between POM+ PVA and POM+pFF. POM is developed by the Research Institute for the Functional Peptides, Yamagata, Japan. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pubmed/18408352 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |