Cell line/type | Whole embryo |
---|---|
Species | Porcine (Pig) |
Animal free | Yes |
Product | TCM-199–HEPES-PVA |
Sanchez-Osorio, J., Cuello, C., Gil, M. A., Parrilla, I., Maside, C., Almiñana, C., ... & Martinez, E. A. (2010). Vitrification and warming of in vivo–derived porcine embryos in a chemically defined medium. Theriogenology, 73(3), 300-308. In this study, a protocol for vitrification and warming of porcine embryos in a chemically defined medium was designed. It was concluded that PVA can be used as a substitute for serum in vitrification and warming solutions without detrimental effects on the in vitro development of in vivo-derived porcine morulae and blastocysts. The composition of TCM-199–HEPES-PVA medium is described in 2.8. Experimental design, experiment 1 & 2, and in Table 1 Effect of PVA added to the vitrification media on the in vitro survival and hatching rates (least squares means SEM) of in vivo–derived porcine embryos (8 replicates). http://doi: 10.1016/j.theriogenology.2009.07.031. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |