|Cell line/type||Whole embryo|
Yoshioka, K. (2011). Development and application of a chemically defined medium for the in vitro production of porcine embryos. Journal of Reproduction and Development, 57(1), 9-16.
In this study, a chemically defined media for IVP of porcine embryos was developed, based on the composition of pig oviductal fluid, analysis of the physical action of substances and improvement of medium components using defined IVP system and in vivo viability of in vitro-produced embryos after transfer to recipients were described.
The concentration of inorganic and energy substrates in PZM is listed in Table 1.https://www.jstage.jst.go.jp/article/jrd/57/1/57_10-196E/_pdf
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > No||No|