|Cell line/type||Whole embryo|
Cao, Z., Sui, L., Li, Y., Ji, S., Zhang, X., & Zhang, Y. (2012). Effects of chemically defined medium on early development of porcine embryos derived from parthenogenetic activation and cloning. Zygote, 20(3), 229-236.
This study investigated if a completely chemically defined medium (PZM-4) could support the early development of porcine embryos derived from parthenogenetic activation (PA) and cloning (somatic cell nuclear transfer, SCNT), and to lay the foundation for determining the physiological roles of certain supplements in this medium. The authors suggest that the completely chemically defined medium PZM-4 can be used to efficiently support the early development of porcine PA and SCNT embryos.
The formulation of PZM-4 media is listed in a former article --> Yoshioka, K., Suzuki, C., Tanaka, A., Anas, I. M. K., & Iwamura, S. (2002). Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biology of reproduction, 66(1), 112-119. See TABLE 1. Composition of culture media.https://www.cambridge.org/core/services/aop-cambridge-core/content/view/F6DF5BFC850AC1DEB2DE3754BE8E64E5/S0967199411000153a.pdf/div-class-title-effects-of-chemically-defined-medium-on-early-development-of-porcine-embryos-derived-from-parthenogenetic-activ
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > No||No|