Cell line/type | Embryonic cells |
---|---|
Species | Mouse |
Animal free | Unspecified |
Product | DMEM-F12 Yamaguchi et al. 2007 |
Yamaguchi, H., Zhu, J., Yu, T., Sasaki, K., Umetsu, H., Kidachi, Y., & Ryoyama, K. (2007). Serum‐free mouse embryo cells generate a self‐sustaining feedback loop for an astrocyte marker protein and respond to cytokines and bisphenol A in accordance with the subtle difference in their differentiation state. Cell biology international, 31(6), 638-644. In this study, serum-free mouse embryo (SFME) cells, the astrocyte progenitor cells in the central nervous system, generated a self-sustaining feedback loop for glial fibrillary acidic protein (GFAP) expression after a period of cell passages. The period required was about 150 days (30 passages). SFME and high-GFAP-expressing SFME (G-SFME) cells were exposed to 10 ng/ml leukemia inhibitory factor (LIF) and 10 ng/ml bone morphogenetic protein 2 (BMP2) to induce differentiation and their responses to cytokine signals were analyzed. Although differentiation was significantly induced in both cell types, SFME cells showed more obvious responses to the cytokine signals. Various concentrations of bisphenol A (BPA) (0.1 pg/ml to 1 microg/ml) were added to determine its effects on cell differentiation. A completely serum-free culture was developed for effective differentiation of G-SFME cells with LIF and BMP2, and GFAP expression was significantly increased in the presence of 1-100 pg/ml BPA. The formulation of the serum-free medium is listed in the Materials and Methods, under the headings; 2.2 Cell culture and 2.3. Assays of cell proliferation. https://onlinelibrary.wiley.com/doi/pdf/10.1016/j.cellbi.2006.11.024 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |