Cell line/type | CD34+ |
---|---|
Species | Human |
Animal free | Unspecified |
Product | HP01 |
Mattia, G., Milazzo, L., Vulcano, F., Pascuccio, M., Macioce, G., Hassan, H. J., & Giampaolo, A. (2008). Long–term platelet production assessed in NOD/SCID mice injected with cord blood CD34+ cells, thrombopoietin–amplified in clinical grade serum–free culture. Experimental hematology, 36(2), 244-252. This study assesses the hypothesis that CB-CD34+ amplification with thrombopoietin (TPO) can be applied to a portion of a CB transplant unit to stimulate recovery along MK differentiation program. Human CB-CD34+ cells were amplified in a serum-free, clinical grade medium with 100 ng/mL TPO alone and in addition to other cytokines (Kit ligand, interleukin-6, and Flt-3 ligand). Seven-day cultured cells were transplanted into irradiated NOD/SCID mice and engraftment, megakaryocytopoiesis, and platelet production was assessed. Platelet release was successful and continuously present for at least 8 weeks in NOD/SCID mice transplanted with CB cells stimulated by TPO. The cells were cultured in serum–free HP01 medium. HP01 is a commecially available medium. The precise formulation is proprietary. https://ac.els-cdn.com/S0301472X07005772/1-s2.0-S0301472X07005772-main.pdf?_tid=bf6dbe58-cc10-4147-b305-b7c69661b912&acdnat=1534314136_f18e34de6aea99e0fd177fa3db10873f |
|
Source | Literature - commercial |
Chemically defined > Unspecified | Unspecified |