Cell line/type | CD34+ |
---|---|
Species | Human |
Animal free | Yes |
Product | X-VIVO 10 |
Clanchy, F. I., & Hamilton, J. A. (2013). The development of macrophages from human CD34+ haematopoietic stem cells in serum-free cultures is optimized by IL-3 and SCF. Cytokine, 61(1), 33-37. In this study, CD34+ cells were cultured in serum-free medium containing Flt3L, SCF, IL-3, IL-6 and M-CSF. Using differing combinations of growth factors, the effect on cell proliferation and differentiation to adherent macrophage-like cells was determined. The proliferative response of CD34+ cells to M-CSF was determined during the initial phase of cell culture. Thirteen combinations of SCF, IL-3, IL-6 and M-CSF were then compared to determine the optimum combination for proliferation. Adherence was used to isolate mature macrophages, and the macrophage-like phenotype was confirmed by analyses of surface markers, histo-morphology and phagocytosis. This study refines the means by which large numbers of macrophages are obtained under serum-free conditions from haematopoietic precursors. Cells were cultured in X-VIVO 10 supplemented with different components as specified in the Materials and Methods, under the heading; 2.1. Isolation and culture of CD34+ cells. X-VIVO 10 is a commercial and proprietary product. https://www.sciencedirect.com/science/article/pii/S1043466612006953 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |