Cell line/type | CD34+ |
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Species | Human |
Animal free | Unspecified |
Product | QBSF-60 |
Hatami, J., Andrade, P. Z., de Matos, A. P. A., Djokovic, D., Lilaia, C., Ferreira, F. C., ... & da Silva, C. L. (2015). Developing a co-culture system for effective megakaryo/thrombopoiesis from umbilical cord blood hematopoietic stem/progenitor cells. Cytotherapy, 17(4), 428-442. In this study, a two-stage culture protocol was exploited aiming to study the effect of different parameters on the megakaryo/thrombopoiesis ex vivo. In the expansion stage, human umbilical cord blood (UCB)-derived CD34(+)-enriched cells were expanded in co-culture with human bone marrow mesenchymal stromal cells (BM-MSCs). The megakaryocytic commitment and platelet generation were studied, considering the impact of exogenous addition of thrombopoietin (TPO) in the expansion stage and a cytokine cocktail (Cyt) including TPO and interleukin-3 in the differentiation stage, with the use of different culture medium formulations, and in the presence/absence of BM-MSCs (direct versus non-direct cell-cell contact). UCB CD34+-enriched cells were seeded to a T25 flask (BD) with the use of QBSF-60 (Quality Biological Inc) as an expansion culture medium at the density of 3.0 × 104cells/mL, over a mitomycin C–treated BM-MSC feeder layer. QBSF-60 is a commercial and proprietary serum-free medium. https://www.sciencedirect.com/science/article/pii/S1465324915000067 |
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Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |