MesenCult™-ACF Culture Kit is a standardized, animal component-free and serum-free system for the isolation and culture of human mesenchymal stem cells (MSCs). It consists of MesenCult™-ACF Medium (Catalog #05440) and MesenCult™-ACF Attachment Substrate (Catalog #05444) and is optimized for the expansion of MSCs in vitro as well as their enumeration using the colony-forming unit-fibroblast (CFU-F) assay. This product supports the long-term growth of human bone marrow- and adipose tissue-derived MSCs as cells maintain robust multi-lineage differentiation potential. MesenCult™-ACF Medium must be used in conjunction with MesenCult™-ACF Attachment Substrate and MesenCult™-ACF Dissociation Kit (Catalog #05426). Components of MesenCult™-ACF Attachment Substrate and MesenCult™-ACF Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Medium. MesenCult™-ACF Medium must be supplemented with L-glutamine.