Cell line/type | MSCs (mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | No |
Product | Serum- medium Battula et al. 2007 |
Battula, V. L., Bareiss, P. M., Treml, S., Conrad, S., Albert, I., Hojak, S., ... & Bühring, H. J. (2007). Human placenta and bone marrow derived MSC cultured in serum‐free, b‐FGF‐containing medium express cell surface frizzled‐9 and SSEA‐4 and give rise to multilineage differentiation. Differentiation, 75(4), 279-291. DOI: 10.1111/j.1432-0436.2006.00139.x In this study, using a novel protocol the authors prepared MSC from BM and non-amniotic placenta (PL) by culture of Ficoll-selected cells in gelatin-coated flasks in the presence of a serum-free, basic fibroblast growth factor (b-FGF)-containing medium that was originally designed for the expansion of human embryonic stem cells (ESC). MSC generated in gelatin-coated flasks in the presence of ESC medium revealed a four-to fivefold higher proliferation rate than conventionally prepared MSC which were grown in uncoated flasks in serum-containing medium. The cells were cultured in a serum-free medium designed to expand human ESC (serum-). This medium was prepared as described (Xu et al., 2001) using Knockoutt DMEM containing 20% Knockoutt serum replacement, 1mM L-glutamine, 0.1mM b-mercaptoethanol, 1% non-essential amino acids and 5 ng/ml human basic fibroblast growth factor. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |