Cell line/type | MSCs (mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | No |
Product | StemPro® MSC Chase et al. 2010 |
Chase, L. G., Lakshmipathy, U., Solchaga, L. A., Rao, M. S., & Vemuri, M. C. (2010). A novel serum-free medium for the expansion of human mesenchymal stem cells. Stem cell research & therapy, 1(1), 8. In this study, the authors developed and tested a serum-free growth medium on human bone marrow-derived MSCs through the investigation of multiple parameters including primary cell isolation, multipassage expansion, mesoderm differentiation, cellular phenotype, and gene-expression analysis. Similar to that achieved with traditional culture medium, human MSCs expanded in serum-free medium supplemented with recombinant human platelet-derived growth factor-BB (PDGF-BB), basic fibroblast growth factor (bFGF), and transforming growth factor (TGF)-beta1 showed extensive propagation with retained phenotypic, differentiation, and colony-forming unit potential. Although the described serum-free MSC culture medium is not free of xenogeneic components, this medium provides a substitute for serum-containing medium for research applications, setting the stage for future clinical applications. The optimized serum-free medium StemPro® MSC SFM is a commercial product. The formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226302/pdf/scrt8.pdf |
|
Source | Literature - commercial |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |