Cell line/type | MSCs (mesenchymal stem cells) |
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Species | Human |
Animal free | Yes |
Product | STK2 Sawada et al. 2010 |
Sawada, R., Yamada, T., Tsuchiya, T., & Matsuoka, A. (2010). Microarray analysis of the effects of serum-free medium on gene expression changes in human mesenchymal stem cells during the in vitro culture. Yakugaku zasshi: Journal of the Pharmaceutical Society of Japan, 130(10), 1387-1393. In this study, the effects of serum-free medium was examined on the gene expression changes in human mesenchymal stem cells (hMSCs) during the in vitro culture using a DNA microarray analysis. hMSCs were cultured with two kinds of medium; 1) MSCGM (contains 10% fetal bovine serum) or 2) STK2 (serum-free medium developed for mesechymal stem cells multiplication), and compared hMSCs proliferation, cell morphology, and gene expression changes until 50 days culture. hMSC proliferation was significantly higher in STK2 medium than in MSCGM medium. The cell morphology of hMSC cultured with STK2 was not significantly changed in 50 days culture. The gene expression changes in hMSCs during the in vitro culture were significantly higher in STK2 than in MSCGM. The formulation of STK2 is proprietary https://www.jstage.jst.go.jp/article/yakushi/130/10/130_10_1387/_pdf |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |