Cell line/type | MSCs (mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | CLI method |
Stehlik, D., Pytlik, R., Rychtrmocova, H., Vesela, R., Kopečný, Z., & Trč, T. (2011). Xenogeneic protein free cultivation of mesenchymal multipotent stromal cells. Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca, 78(6), 537-543. The aim of this study was to compare the standard laboratory method of cultivation of mesenchymal multipotent stromal cells (MSC) and a novel technique of rapid MSC expansion focused on simple clinical use. The new animal protein-free method (CLI) was based on the clinical grade medium CellgroTM, pooled human serum and human recombinant growth factors (EGF, PDGF-BB, M-CSF, FGF-2) supplemented with dexamethasone, insulin and ascorbic acid. CLI method is unique, rapid, simple and lacking the addition of animal proteins. CLI-cultured cells fulfill the criteria of MSC. The CLI method potentially allows for closed system cultivation in good manufacturing practice (GMP) conditions. It seems to be easily transferable to good clinical practice compared to other protocols and should extend the possibilities of cell therapy and tissue engineering of cartilage and bone. The CLI method is protected by Czech patent 301 148 and by Europian patent EP 1999250. https://www.ncbi.nlm.nih.gov/pubmed/22217407 |
|
Source | Literature - own formulation |
Chemically defined > No | No |