Cell line/type | MSCs (mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | BM2-SCC |
Díez, J. M., Bauman, E., Gajardo, R., & Jorquera, J. I. (2015). Culture of human mesenchymal stem cells using a candidate pharmaceutical grade xeno-free cell culture supplement derived from industrial human plasma pools. Stem cell research & therapy, 6(1), 28. In this study, cell growth was evaluated by culturing hMSCs with commercial media or media supplemented with an industrial, xeno-free, pharmaceutical-grade supplement for cell culture (SCC) under development at Grifols or fetal bovine serum. SCC supported the growth of commercial hMSCs. Starting from the same number of seeded cells in two consecutive passages of culture with medium supplemented with SCC, hMSC yield and cell population doubling time were equivalent to the values obtained with the commercial medium and was consistent among lots. The viability of hMSCs was higher than 90%, while maintaining the characteristic phenotype of undifferentiated hMSCs (positive for CD29, CD44, CD90, CD105, CD146, CD166 and Stro-1; negative for CD14 and CD19). Cultured hMSCs maintained the potential for differentiation into adipocytes, chondrocytes, osteoblasts, and neurons. The formulation of the basal culture medium BM2 is listed in a previous article, Henríquez et al. 2013, in Table 1. |
|
Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |