Cell line/type | MSCs (mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | DMEM-PRC Samuel et al. 2017 |
Samuel, S., Ahmad, R. E., Ramasamy, T. S., Karunanithi, P., Naveen, S. V., & Kamarul, T. (2017). Platelet-rich concentrate in serum-free medium enhances cartilage-specific extracellular matrix synthesis and reduces chondrocyte hypertrophy of human mesenchymal stromal cells encapsulated in alginate. Platelets, 1-9. In this study, the effect of platelet-rich concentrate (PRC) alone in enhancing the chondrogenic differentiation potential of human mesenchymal stromal cells (hMSC) encapsulated in three-dimensional alginate constructs, was investigated. Cells encapsulated in alginate were cultured in serum-free medium supplemented with only 15% PRC. Results showed that the cells cultured in the presence of PRC for 24 days maintained a chondrocytic phenotype and demonstrated minimal upregulation of cartilaginous extracellular matrix (ECM) marker genes (SOX9, TNC, COL2, ACAN, COMP) and reduced expression of chondrocyte hypertrophy genes (Col X, Runx2) compared to the standard chondrogenic medium (p < 0.05). PRC group had correspondingly higher levels of glycosaminoglycan and increased concentration of chondrogenic specific proteins (COL2, ACAN, COMP) in the ECM. IPRC appears to be equally as effective as the commercially available chondrogenic medium in enhancing chondrogenesis of the hMSC. PRC also renders benefit in delaying chondrocyte hypertrophy in the differentiated cells. The formulation of DMEM-PRC medium is specified in the Methods, under the heading; In vitro cell proliferation assay. https://www.tandfonline.com/doi/full/10.1080/09537104.2017.1371287 |
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Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |