Cell line/type | MSCs (mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | SFM-XF |
Chase, L. G., Yang, S., Zachar, V., Yang, Z., Lakshmipathy, U., Bradford, J., ... & Vemuri, M. C. (2012). Development and characterization of a clinically compliant xeno‐free culture medium in good manufacturing practice for human multipotent mesenchymal stem cells. Stem cells translational medicine, 1(10), 750-758. In this study, the authors developed and tested a serum-free and xeno-free culture medium (SFM-XF) using human bone marrow- and adipose-derived MSCs by investigating primary cell isolation, multiple passage expansion, mesoderm differentiation, cellular phenotype, and gene expression analysis, which are critical for complying with translation to cell therapy. This study reports the development of such a GMP-manufactured xeno-free culture medium with a proprietary mix of proteins, including human serum albumin, lipoproteins, recombinant human growth factors, and trace elements that are free of any nonhuman or animal origin components, and thus sets the stage for future MSC therapeutic applications. SFM-XF culture system allows better expansion and multipotentiality of MSCs and serves as a preferred alternative to serum-containing media for the production of large-scale, functionally competent MSCs for future clinical applications. The precise formulation of SFM-XF is proprietary.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659659/pdf/sct750.pdf |
|
Source | Literature - commercial |
Chemically defined > Unspecified | Unspecified |