Cell line/type | MSCs (mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | No |
Product | PPRF-msc6 |
Jung, S., Sen, A., Rosenberg, L., & Behie, L. A. (2012). Human mesenchymal stem cell culture: rapid and efficient isolation and expansion in a defined serumāfree medium. Journal of tissue engineering and regenerative medicine, 6(5), 391-403. This study provides evidence demonstrating that a defined serum-free medium (PPRF-msc6) shows several beneficial features over a conventional FBS-containing medium for the production of hMSCs. When compared to control FBS-based cultures, PPRF-msc6 medium supported the derivation of hMSCs from primary cultures of bone marrow cells in a more rapid and consistent manner. Furthermore, hMSCs cultured in PPRF-msc6 exhibited: (a) a greater colony-forming capacity in primary as well as passaged cultures; (b) negligible lag phase and explicit exponential growth; (c) lower population doubling times (21-26 h vs 35-38 h between passage levels 1 and 10); (d) a greater number of population doublings (62 +/- 4 vs 43 +/- 2; over a 2 month period); and (e) a higher degree of homogeneity in size. The formulation of PPRF-msc6 medium is listed in a previous article, Jung et al. 2010. Jung, S., Sen, A., Rosenberg, L., & Behie, L. A. (2010). Identification of growth and attachment factors for the serum-free isolation and expansion of human mesenchymal stromal cells. Cytotherapy, 12(5), 637-657. https://onlinelibrary.wiley.com/doi/pdf/10.1002/term.441 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |