Cell line/type | MSCs (mesenchymal stem cells) |
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Species | Human |
Animal free | Yes |
Product | STK2 Lee et al. 2017 |
Lee, M. S., Youn, C., Kim, J. H., Park, B. J., Ahn, J., Hong, S., ... & Park, S. G. (2017). Enhanced cell growth of adipocyte-derived mesenchymal stem cells using chemically-defined serum-free media. International Journal of Molecular Sciences, 18(8), 1779. In this study, adipose-derived MSCs showed a significantly higher proliferation rate in STK2, a chemically-defined medium, than in DMEM/FBS. The expression of MSC surface markers was increased in the culture using STK2 compared to that using DMEM/FBS. Tri-lineage differentiation analyses showed that MSCs cultured in STK2 were superior to those cultured in DMEM/FBS. In addition, MSCs cultured in STK2 showed a reduced senescence rate, small and homogenous cell size, and were more genetically stable compared to those cultured in DMEM/FBS. Furthermore, secretome analysis showed that the expression of factors related to proliferation/migration, anti-inflammation, and differentiation were increased in STK2 culture medium compared to DMEM/FBS. The precise formulation of STK2 medium is proprietary. https://res.mdpi.com/def502000e1462764c97dcf3e8bb20feebd72b5cd304ea7e7d6d417e4aff20d2f46caecec24eedc3c8ccd263653a704cd100d853d60858cee51dc68412ebf87a6c0e07d8bf3c70fd1b9b592ccb0c8d288c1d1aed82d7ed9bb1eb650d98020164a821d7773f015039d857a3adb1b6fe7e2db7c69fdb |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |