Cell line/type | MSCs (mesenchymal stem cells) |
---|---|
Species | Equine (Horse) |
Animal free | No |
Product | ePL media |
Naskou, M. C., Sumner, S. M., Chocallo, A., Kemelmakher, H., Thoresen, M., Copland, I., ... & Peroni, J. F. (2018). Platelet lysate as a novel serum-free media supplement for the culture of equine bone marrow-derived mesenchymal stem cells. Stem cell research & therapy, 9(1), 75. In this study, the use of equine platelet lysate (ePL) pooled from donor horses in place of FBS to culture equine MSCs was evaluated. The results showed that MSCs cultured in ePL media exhibited similar proliferation rates (PD and DT) compared with those cultured in FBS at individual time points. MSCs cultured in ePL showed a statistically significant increased viability following a single washing step, expressed similar levels of MSC markers compared to FBS, and were able to differentiate towards the three lineages. Finally, MSCs cultured in ePL efficiently suppressed the release of TNF-α when exposed to LPS-stimulated monocytes similar to those cultured in FBS. The formulation of ePL media is listed in the Methods, under the heading; Isolation and culture of equine bone marrow-derived MSCs with FBS or ePL media supplement. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5863827/pdf/13287_2018_Article_823.pdf |
|
Source | Literature - own formulation |
Chemically defined > No | No |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |