Cell line/type | Keratinocytes |
---|---|
Species | Human |
Animal free | Yes |
Product | VN:GF medium |
Richards, S., Leavesley, D., Topping, G., & Upton, Z. (2008). Development of defined media for the serum-free expansion of primary keratinocytes and human embryonic stem cells. Tissue Engineering Part C: Methods, 14(3), 221-232. In this study, the aim was to determine whether primary Kc and hES cells can be established and serially propagated serum-free using medium containing VN, insulin-like growth factor-I, and insulin-like growth factor binding protein-3 (VN:GF). Here we demonstrate that primary Kc cells can be isolated, established, serially propagated, and re-form an epidermal layer using the VN:GF combination. Additionally, cell proliferation studies indicate that the Kcs proliferate using the VN:GF combination at a rate comparable to cells grown using serum. Similarly, we verified that this VN:GF combination could be employed for the serial propagation of hES cells. Importantly, both the Kc and hES cells retain their undifferentiated phenotype when cultured using the VN:GF combinations as a serum-free medium for up to 4 passages for Kc and at least 10 passages for hES cells as indicated by the expression of a range of cell surface markers. This study demonstrates that the novel, fully defined VN:GF medium is a viable alternative to media containing serum and highlights the potential of this technology for generating therapeutically viable cells and tissues. The formulation of the VN:GF medium is listed in the Materials and Methods, under the heading; Kcs cultured in VN:GF medium. https://www.liebertpub.com/doi/abs/10.1089/ten.tec.2007.0428?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |