Cell line/type | Keratinocytes |
---|---|
Species | Human |
Animal free | Yes |
Product | Defined Keratinocyte-SFM/ Epidermal Keratinocyte 3D Medium |
Ng, W., & Ikeda, S. (2011). Standardized, defined serum-free culture of a human skin equivalent on fibroblast-populated collagen scaffold. Acta dermato-venereologica, 91(4), 387-391. In this study, the authors assessed the use of standardized, serum-free and bovine pituitary extract-free keratinocyte culture media in the development of a human skin equivalent. After culture at the air-liquid interface for 3 weeks on a fibroblast-populated collagen lattice, an orthokeratinized, pluristratified epithelium was produced which expressed cytokeratins, cornified cell envelope precursors (involucrin, transglutaminase 1, filaggrin) and desmosomal components (desmoglein and desmocollin 1 and 3, plakophilin 1) in a differentiation-specific manner. There was also evidence of basement membrane reconstitution with collagen IV/VII, laminin 5, alpha6 and beta4 integrin subunit expression at the epithelial-matrix junction. Overall, the findings demonstrate that readily available, defined organotypic culture media can be used to generate a reproducible skin equivalent with hallmarks of epidermal differentiation. In this study, the reconstituted epithelium in defined media displayed a close histological resemblance to the native epidermis as early as one week after air-exposure, and a well-organized epidermal architecture was maintained after 4 weeks of culture at the air-liquid interface. Defined Keratinocyte Serum Free Medium and Epidermal Keratinocyte 3D Medium are both commercial products. The precise formulation is proprietary. https://pdfs.semanticscholar.org/2143/8179badecef73e28c4621f4f6fe847224168.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |