Cell line/type | Keratinocytes |
---|---|
Species | Human |
Animal free | Yes |
Product | HECK 109 |
Wille, J. J., Burdge, J. J., & Park, J. Y. (2014). Methods for the preparation of an autologous serum-free cultured epidermis and for autografting applications. In Epidermal Cells (pp. 203-218). Springer, New York, NY. In this study culture techniques required for the formation of a hole-free monolayer of undifferentiated basal keratinocytes without the use of an organotypic matrix substrate are accomplished with a specially designed nutrient basal media (HECK 109) that is a chemically defined and subsequent culture in this serum-free culture media supplemented with hormones and two human recombinant protein growth factors (EGF and IGF-1). The formulation of the basal nutrient medium HECK-109 is listed in Table 1. Preparation of HECK 109 Serum-Free Medium is described in the Materials and Methods. The preparation of HECK 109 serum-free medium is the subject of a patent issued to the author (J.J.W.). U.S. Patent: 5,686,307 (11/11/97) issued to J.J. Wille discloses the composition of HECK 109 serum-free media for preparing a CEA graft. https://link.springer.com/content/pdf/10.1007%2F7651_2014_72.pdf |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |