Cell line/type | HEK-293 (RRID:CVCL_6F04) |
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Species | Human |
Animal free | Yes |
Product | DMEM Jaluria et al. 2008 |
Jaluria, P., Konstantopoulos, K., Betenbaugh, M., & Shiloach, J. (2008). Egr1 and Gas6 facilitate the adaptation of HEK‐293 cells to serum‐free media by conferring enhanced viability and higher growth rates. Biotechnology and bioengineering, 99(6), 1443-1452. In this study, to better understand the physiological responses associated with serum withdrawal and to expedite the adaptation process, a Human Embryonic Kidney-293 (HEK-293) cell line was propagated in 10% fetal bovine serum (FBS) and was progressively adapted to SFM and analyzed at specific serum levels by oligonucleotide microarrays. HEK-293 cells, propagated in 10% FBS were transfected with egr1 or gas6 and then adapted to SFM. Results indicated that higher expression of either gene moderately enhanced the ability of both cell lines to adapt to SFM. These results indicate that enhanced expression of egr1 or gas6 facilitate adaptation to SFM by improving growth and viability. These cells were initially grown using DMEM containing glutamine supplemented with 10% fetal bovine serum. Cells were adapted to SFM gradually using a sequential method, whereby cells were slowly transferred into complete media containing reduced levels of serum. The protocol for serum-free adaptation is described in the mentioned reference Sinacore MS et a. 2000. Sinacore MS, Drapeau D, Adamson SR. 2000. Adaptation of mammalian cells to growth in serum-free media. Mol Biotechnol 15:249–257. https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.21707 |
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Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |