Cell line/type | HEK-293 (RRID:CVCL_6F04) |
---|---|
Species | Human |
Animal free | Yes |
Product | F17 medium |
Mignaqui, A. C., Ruiz, V., Perret, S., St-Laurent, G., Chahal, P. S., Transfiguracion, J., ... & Wigdorovitz, A. (2013). Transient gene expression in serum-free suspension-growing mammalian cells for the production of foot-and-mouth disease virus empty capsids. PloS one, 8(8), e72800. In this study, transient gene expression (TGE) in serum-free suspension-growing mammalian cells was explored for the production of FMDV recombinant empty capsids as a subunit vaccine. The human embryonic kidney 293 cell line stably expressingba truncated Epstein–Barr virus Nuclear Antigen-1 (293-6E) was grown in suspension in a serum-free F17 medium. F17 medium is a commercially available product. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748020/pdf/pone.0072800.pdf |
|
Source | Literature - commercial |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |