Cell line/type | iPSc (induced Pluripotent Stem Cells) |
---|---|
Species | Mouse |
Animal free | No |
Product | iPSC medium |
Hirai, H., Katoku-Kikyo, N., Karian, P., Firpo, M., & Kikyo, N. (2012). Efficient iPS cell production with the MyoD transactivation domain in serum-free culture. PloS one, 7(3), e34149. In this study, the efficiency was raised of making mouse iPSCs with M(3)O-SKM to 26% by culturing transduced cells at low density in serum-free culture medium. In contrast, the efficiency increased from 0.1% to only 2% with the combination of wild-type Oct4 and SKM (OSKM) under the same culture condition. For human iPSCs, M(3)O-SKM achieved 7% efficiency under a similar serum-free culture condition, in comparison to 1% efficiency with OSKM. The formulation of the iPSC medium, a DMEM-KSR based medium is listed in the Materials and Methods, under the heading; Preparation of iPSCs from MEFs. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316619/pdf/pone.0034149.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |