AggreWell™ EB Formation Medium is a defined, serum-free medium that supports the survival of mTeSR™1-, TeSR™2-, or TeSR™-E8™-cultured human embryonic stem (ES) cells or human induced pluripotent stem (iPS) cells during generation and subsequent culture of embryoid bodies (EBs). This medium contains a reduced level of bFGF; this allows it to be used as a base medium to which specific growth factors can be added for differentiation protocols.