Cell line/type | iPSc (induced Pluripotent Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | F44m / XF-hESm |
Rodríguez‐Pizà, I., Richaud‐Patin, Y., Vassena, R., González, F., Barrero, M. J., Veiga, A., ... & Belmonte, J. C. I. (2010). Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno‐free conditions. Stem Cells, 28(1), 36-44. In this study, a protocol was developed to generate and maintain human iPSC that would be entirely devoid of xenobiotics. A xeno-free cell culture media was developed that supported the long-term propagation of human embryonic stem cells (hESCs) to a similar extent as conventional media containing animal origin products or commercially available xeno-free medium. Primary cultures of human dermal fibroblasts were derived under strict xeno-free conditions (XF-HFF), that can be used as both the cell source for iPSC generation as well as autologous feeder cells to support their growth.Other reagents of animal origin (trypsin, gelatin, matrigel) are replaced with their recombinant equivalents. Finally, vesicular stomatitis virus G-pseudotyped retroviral particles expressing a polycistronic construct encoding Oct4, Sox2, Klf4, and GFP were used to reprogram XF-HFF cells under xeno-free conditions. A total of 10 xeno-free human iPSC lines were generated, which could be continuously passaged in xeno-free conditions and maintained characteristics indistinguishable from hESCs, including colony morphology and growth behavior, expression of pluripotency-associated markers, and pluripotent differentiation ability in vitro and in teratoma assays. Overall, the results presented here demonstrate that human iPSCs can be generated and maintained under strict xeno-free conditions and provide a path to good manufacturing practice (GMP) applicability that should facilitate the clinical translation of iPSC-based therapies. The formulation of the serum-free media in this study, F44m and XF-hESm are listed in the Materials and Methods, under the heading; Media for hESC/iPSC Culture. https://stemcellsjournals.onlinelibrary.wiley.com/doi/pdf/10.1002/stem.248 |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |