Cell line/type | iPSc (induced Pluripotent Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | mTeSR1 Mohamad et al. 2013 |
Mohamad, O., Drury-Stewart, D., Song, M., Faulkner, B., Chen, D., Yu, S. P., & Wei, L. (2013). Vector-free and transgene-free human iPS cells differentiate into functional neurons and enhance functional recovery after ischemic stroke in mice. PLoS One, 8(5), e64160. In this study, vector-free hiPS cells were maintained in feeder-free and serum-free conditions and differentiated into functional neurons in vitro using a newly developed differentiation protocol. Twenty eight days after transplantation in stroke mice, hiPS-NPCs showed mature neuronal markers in vivo. No tumor formation was seen up to 12 months after transplantation. Transplantation of hiPS-NPCs restored neurovascular coupling, increased trophic support and promoted behavioral recovery after stroke. These data suggest that using vector-free and transgene-free hiPS cells in stem cell therapy are safe and efficacious in enhancing recovery after focal ischemic stroke in mice. hiPS cells were maintained in feeder- and serum-free media, mTeSR1. mTeSR1 is a commercially avialable product. The precise formulation is proprietary.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662762/pdf/pone.0064160.pdf |
|
Source | Literature - commercial |
Chemically defined > Yes | Yes |