Cell line/type | iPSc (induced Pluripotent Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | X medium |
Wang, J., Hao, J., Bai, D., Gu, Q., Han, W., Wang, L., ... & Liu, Z. (2015). Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions. Stem cell research & therapy, 6(1), 223. In this study, clinical-grade human foreskin fibroblast (HFF) cells used as feeder cells and parental cells of the clinical-grade hiPSCs were isolated from human foreskin tissues and cultured in Xeno-free media. Clinical-grade hiPSCs were derived by integration-free Sendai virus-based reprogramming kit in Xeno-free pluriton reprogramming medium or X medium. Several integration-free clinical-grade hiPSC lines were successfully derived under GMP-controlled conditions and with Xeno-free reagents culture media in line with the current guidance of international and national evaluation criteria. The formulation of X medium is listed in the Methods, under the heading; Cell culture. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643509/pdf/13287_2015_Article_206.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |