Cell line/type | iPSc (induced Pluripotent Stem Cells) |
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Species | Human |
Animal free | Yes |
Product | mTeSR1-ROCK inhibitor |
Miranda, C. C., Fernandes, T. G., Diogo, M. M., & Cabral, J. M. (2016). Scaling up a chemically‐defined aggregate‐based suspension culture system for neural commitment of human pluripotent stem cells. Biotechnology journal, 11(12), 1628-1638. In this study, the scaling-up of an aggregate-based culture system for neural induction of human induced pluripotent stem cells (hiPSCs) under chemically-defined conditions is described. A combination of non-enzymatic dissociation and rotary agitation was successfully used to produce homogeneous populations of hiPSC aggregates with an optimal (140 mum) and narrow distribution of diameters (coefficient of variation of 21.6%). mTeSR1 medium was supplemented with different components as specified in the Materials and Methods, under the heading; 2.2 Culture of hiPSCs in 3D conditions. mTeSR1 medium is a commercial product. The formulation is proprietary. https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.201600446 |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |