Cell line/type | iPSc (induced Pluripotent Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | CDM-PVA |
Soares, F. A., Chandra, A., Thomas, R. J., Pedersen, R. A., Vallier, L., & Williams, D. J. (2014). Investigating the feasibility of scale up and automation of human induced pluripotent stem cells cultured in aggregates in feeder free conditions. Journal of biotechnology, 173, 53-58. This study describes the first published protocol for scalable automated expansion of human induced pluripotent stem cell lines growing in aggregates in feeder-free and chemically defined medium. Cells were successfully transferred between different sites representative of research and manufacturing settings; and passaged manually and using the CompacT SelecT automation platform. Modified protocols were developed for the automated system and the management of cells aggregates (clumps) was identified as the critical step. Cellular morphology, pluripotency gene expression and differentiation into the three germ layers have been used compare the outcomes of manual and automated processes. The formula is listed in the Materials and Methods, under the heading; 2.1. Manual maintenance and passage of hiPSC in feeder free and chemically defined medium. https://www.sciencedirect.com/science/article/pii/S016816561300552X |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Antibiotics free > Yes | Yes |
Contains phenol red > No | No |