Cell line/type | iPSc (induced Pluripotent Stem Cells) |
---|---|
Species | Human |
Animal free | Unspecified |
Product | mTeSR1 Alamein et al. 2015 |
Alamein, M. A., Wolvetang, E. J., Ovchinnikov, D. A., Stephens, S., Sanders, K., & Warnke, P. H. (2015). Polymeric nanofibrous substrates stimulate pluripotent stem cells to form three‐dimensional multilayered patty‐like spheroids in feeder‐free culture and maintain their pluripotency. Journal of tissue engineering and regenerative medicine, 9(9), 1078-1083. In this study, the authors show that electrospun polymer matrices comprised of nanofibres that mimic the architecture of the natural fibrous extracellular matrix allow for feeder-free expansion of pluripotent human induced pluripotent stem cells (IPSCs) and human embryonic stem cells (HESCs) into multilayered 3D ’patty-like’ spheroid structures in a defined xeno-free culture medium. mTeSR1 medium is a commercial product. The precise formulation is proprietary. https://onlinelibrary.wiley.com/doi/pdf/10.1002/term.1960 |
|
Source | Literature - commercial |
Chemically defined > Yes | Yes |