Cell line/type | iPSc (induced Pluripotent Stem Cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | E8 medium |
Beers, J., Linask, K. L., Chen, J. A., Siniscalchi, L. I., Lin, Y., Zheng, W., ... & Chen, G. (2015). A cost-effective and efficient reprogramming platform for large-scale production of integration-free human induced pluripotent stem cells in chemically defined culture. Scientific reports, 5, 11319. In this study a cost-effective and efficient reprogramming platform was built for production of a large number of integration-free iPSCs in chemically defined medium. Optimization procedure that enables a single technician to make 20-40 lines at a time in a 24-96 well format in a reliable and reproducible fashion is described. The formulation of E8 medium and the protocol for its preparation is listed in a previous article, Sharma et al. 2015. Sharma, A., Li, G., Rajarajan, K., Hamaguchi, R., Burridge, P. W., & Wu, S. M. (2015). Derivation of highly purified cardiomyocytes from human induced pluripotent stem cells using small molecule-modulated differentiation and subsequent glucose starvation. Journal of visualized experiments: JoVE, (97). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4464084/pdf/srep11319.pdf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |