Cell line/type | ESCs (Embryonic Stem Cells) |
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Species | Mouse |
Animal free | No |
Product | high glucose DMEM-KSR Alfred et al. 2010 |
Alfred, R., Gareau, T., Krawetz, R., Rancourt, D., & Kallos, M. S. (2010). Serumāfree scaled up expansion and differentiation of murine embryonic stem cells to osteoblasts in suspension bioreactors. Biotechnology and bioengineering, 106(5), 829-840. In this study, the authors report efficient adaptation of mESCs to serum-free medium in static and subsequent scale-up production of mESC derived osteoblasts in suspension bioreactors. Murine ESCS were weaned from serum into serum-free media in a multi-step process with a 3% reduction in FBS, and a corresponding increase in KSR at each step (passage). It was observed that mESC colonies cultured in 15% KSR medium in static culture developed a more rounded morphology compared to 15% FBS supplemented medium. Additionally, cells cultured in 15% KSR attained the highest peak cell densities on day 6 (144 h) compared to cells cultured in all other medium types. Morphology shows increased aggregation when murine embryonic stem cells were cultured in 15% KSR compared to 15% FBS cultures. The formulation of the serum-free high glucose DMEM-KSR based medium is listed in the Methods, under the heading; Culture Medium & Murine Embryonic Stem Cell Expansion in Static Culture. https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.22727 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |