Cell line/type | ESCs (Embryonic Stem Cells) |
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Species | Mouse |
Animal free | Unspecified |
Product | ESGRO complete medium and KO-DMEM/SR supplemented |
Fernandes, T. G., Fernandes-Platzgummer, A. M., Da Silva, C. L., Diogo, M. M., & Cabral, J. M. (2010). Kinetic and metabolic analysis of mouse embryonic stem cell expansion under serum-free conditions. Biotechnology letters, 32(1), 171. The influence of the initial cell density of mouse ES cells on cell expansion was assessed. Concomitantly, the biochemical profile of the culture was evaluated, which allowed measuring the consumption of important substrates, such as glucose and glutamine, and the production of metabolic byproducts, like lactate. The results suggest a more efficient cell metabolism in serum-free conditions and a preferential use of glutaminolysis as an energy source during cell expansion at low seeding densities. This work contributes to the development of fully-controlled bioprocesses to produce relevant numbers of ES cells for cell therapies and high-throughput drug screening. Direct comparison of serum-containing medium supplemented with LIF and serum-free medium (both ESGRO complete and DMEM/SR with LIF) did not show significant differences in average fold increase and specific growth rate values for each seeding density used. This result implies that mouse ES cell expansion kinetics was not affected by the absence of FBS. In fact, the combination of LIF and BMP-4, in the case of ESGRO complete medium, or LIF with serum-replacement factors, for DMEM/SR medium, provided sufficient signals for cell self-renewal and proliferation, namely by activating STAT-3 and ID proteins. Overall, these results demonstrate that cells expanded in serum-free conditions retain their pluripotency when proper signals are exogenously supplemented to the culture medium. ESGRO complete medium and KO-DMEM/SR are supplemented with different components as specified in the Materials and Methods. ESGRO complete medium and Knockout DMEM are commercial products. The precise formulation is proprietary. https://link.springer.com/content/pdf/10.1007%2Fs10529-009-0108-0.pdf |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |