Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Mouse |
Animal free | No |
Product | (high glucose) DMEM-KSR Alfred et al. 2011 |
Alfred, R., Taiani, J. T., Krawetz, R. J., Yamashita, A., Rancourt, D. E., & Kallos, M. S. (2011). Large-scale production of murine embryonic stem cell-derived osteoblasts and chondrocytes on microcarriers in serum-free media. Biomaterials, 32(26), 6006-6016 In this study, serum-free protocols are presented for the successful production of murine embryonic stem cell (mESC) derived osteoblasts and chondrocytes on CultiSpher S macroporous microcarriers in stirred suspension bioreactors. e. In addition, the ESCs maintained their pluripotency after long-term culture (28 days) in serum-free medium. This is the first time mESCs have been cultured on microcarriers without prior exposure to serum and/or fibroblasts, while also eliminating the excessive agglomeration plaguing earlier studies. These protocols provide an economical, practical, serum-free means for expanding ESCs in a stirred suspension bioprocess. The formulations of the high glucose DMEM-KSR and DMEM-KSR based Maintenance medium, Osteogenic medium and Chondrogenic medium are listed in the Materials and Methods under the heading; 2.2. Culture medium. https://ac.els-cdn.com/S0142961211003978/1-s2.0-S0142961211003978-main.pdf?_tid=ca5e49b8-eb3e-4861-82a7-9e27f35be04f&acdnat=1533799376_99e2e973f28de38e6a99b1167c4f21cf |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |