Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Mouse |
Animal free | No |
Product | ESF7 medium Li et al. 2011 |
Li, Y., Yokohama-Tamaki, T., & Tanaka, T. S. (2011). Short-term serum-free culture reveals that inhibition of Gsk3β induces the tumor-like growth of mouse embryonic stem cells. PLoS One, 6(6), e21355. In this study, evidence is presented that the tumor-like growth of mouse embryonic stem cells (mESCs) is suppressed by short-term serum-free culture, which is reversed by pharmacological inhibition of Gsk3beta. Mouse ESCs cultured under chemically-defined serum-free (CDSF) medium conditions maintained the expression of Oct3/4, Nanog, Sox2 and SSEA1, and differentiated into germ cells in vivo. In addition, when mESCs were cultured under CDSF conditions supplemented with FBS, or when the cells were cultured under CDSF conditions followed by standard culture conditions, they consistently developed into teratomas. Thus, these results validate that the pluripotency of mESCs was not compromised by CDSF conditions. The formulation of the chemically-defined serum-free medium ESF7 medium is listed in a previous article, Furue et al. 2005. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |