Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Mouse |
Animal free | No |
Product | DMEM/F12-Neurobasal Wongpaiboonwattana et al. |
Wongpaiboonwattana, W., & Stavridis, M. P. (2015). Neural differentiation of mouse embryonic stem cells in serum-free monolayer culture. Journal of visualized experiments: JoVE, (99). In this study, a method is described for the differentiation of ESC to neural progenitors using serum-free, monolayer culture. The method is scalable, efficient and results in production of ~70% neural progenitor cells within 4 - 6 days. The cells are cultured in a mix of DMEM/F12 medium supplemented with modified N2 supplement and Neurobasal medium supplemented with B27 supplement (1:1). Detailed instructions on media preparation and cell density optimization is provided in the Protocol, under the heading; Protocol Media Preparation. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542683/ |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > Yes | Yes |