|Cell line/type||ESCs (Embryonic Stem Cells)|
|Product||Modified N2B27 media|
Purpura, K. A., Morin, J., & Zandstra, P. W. (2008). Analysis of the temporal and concentration-dependent effects of BMP-4, VEGF, and TPO on development of embryonic stem cell–derived mesoderm and blood progenitors in a defined, serum-free media. Experimental hematology, 36(9), 1186-1198.
The objective of this study was to develop a robust serum-free (SF) system for generation of hemogenic mesoderm and blood progenitors from pluripotent cells. This article describes modifications to the N2B27 media to amplify mesoderm induction and extends earlier work defining blood progenitor cell induction from ESC with BMP-4, VEGF, and TPO.
The formula of N2B27 can be found online. --> http://cshprotocols.cshlp.org/content/2017/5/pdb.rec096131.full?text_only=true. The adaptations are listed inlisted in the Materials and Methods, under the headings Maintenance of ESCs and ESC differentiation.https://www.ncbi.nlm.nih.gov/pubmed/18550259
|Source||Literature - own formulation|
|Chemically defined > Yes||Yes|
|Contains phenol red > No||No|
|Antibiotics free > Yes||Yes|