Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | No |
Product | CDM Pick et al. 2007 |
Pick, M., Azzola, L., Mossman, A., Stanley, E. G., & Elefanty, A. G. (2007). Differentiation of human embryonic stem cells in serumāfree medium reveals distinct roles for bone morphogenetic protein 4, vascular endothelial growth factor, stem cell factor, and fibroblast growth factor 2 in hematopoiesis. Stem Cells, 25(9), 2206-2214. In this study, the authors utilized a serum- and stromal cell-free "spin embryoid body (EB)" differentiation system to investigate the roles of four growth factors, bone morphogenetic protein 4, vascular endothelial growth factor , stem cell factor, and basic fibroblast growth factor, singly and in combination, on the generation of hematopoietic cells from human embryonic stem cells (HESCs). These data demonstrate the utility of the serum-free spin EB system in dissecting the roles of specific growth factors required for the directed differentiation of HESCs toward the hematopoietic lineage. The serum-free chemically defined medium (CDM) used in this study is initially described by Johansson et al. 1995. and modified as described in Ng et al. 2005. |
|
Source | Literature - own formulation |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |