Cell line/type | ESCs (Embryonic Stem Cells) |
---|---|
Species | Human |
Animal free | No |
Product | ko-SR-containing medium Rajala et al. 2007 |
Rajala, K., Hakala, H., Panula, S., Aivio, S., Pihlajamäki, H., Suuronen, R., ... & Skottman, H. (2007). Testing of nine different xeno-free culture media for human embryonic stem cell cultures. Human Reproduction, 22(5), 1231-1238. In this study, nine xeno-free culture media were compared with the conventional serum replacement (ko-SR) containing media in the culture of hESC on human feeder cells. Cultured hESC were characterized immunocytochemically and by fluorescence-activated cell sorter analysis. The differentiation potential of hESC cultured with xeno-free media was determined with the RT-PCR analysis. The hESC cultured in xeno-free media differentiated or the proliferation decreased substantially. Under some test conditions, the morphology of the feeder cells was altered considerably. The hESC cultured with human serum underwent excessive differentiation in the beginning of culture, but a fraction of hESC was able to adapt to culture conditions containing 20% of human serum. None of the studied xeno-free media was able to maintain the undifferentiated growth of hESC. The medium containing 20% human serum was found to sustain undifferentiated hESC proliferation to some extent, yet was inferior to the conventional ko-SR-containing medium. The control ko-SR-containing medium contained 80% (vol/vol) KnockOut DMEM and 20% (vol/vol) ko-SR supplemented with 2 mM Glutamax, 0.1 mM b-mercaptoethanol, 0.1 mM MEM non-essential amino acids, 50 U penicillin/ml–50 mg streptomycin/ml and recombinant human bFGF at 8 ng/ml. https://www.ncbi.nlm.nih.gov/pubmed/17251355 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |